Synthesis and characterization of Erythromycin loaded PLGA and PCL microspheres: Antimicrobial application

Bennabi, Lamia; Abedlmalek, Ilham; Ammari, Abedelkader; Sediri, Khaldia; Bennabi, Fatima; Guemra, Kaddour

doi:10.21608/mb.2022.122642.1051

Synthesis and characterization of Erythromycin loaded PLGA and PCL microspheres: Antimicrobial application

Document Type : Original Article

Authors

¹ Laboratory of Organic Chemistry, Physics, and Macromolecules (LCOPM), University of Sidi Bel Abbes, Sidi Bel Abbes, Algeria.

² Department of Chemistry, Faculty of Matter Sciences, University of Tiaret, Tiaret, Algeria.

³ Laboratory of Micro and Nanophysics (LaMiN), National Polytechnic School of Oran (ENPO), Oran, Algeria.

⁴ Laboratory of Applied Chemistry (LAC), University of Ain Temouchent, Ain Temouchent, Algeria.

⁵ Department of Medicine, University of Saad Dahlb, Blida, Algeria.

10.21608/mb.2022.122642.1051

Abstract

We present in this work the preparation of several formulations based on biocompatible biodegradable polymers: polycaprolactone (PCL) and poly (lactic-co-glycolic) acid (PLGA) loaded with Erythromycin(ERYT) antibiotic. These biocompatible materials were used to pre-pare microspheres with and without immobilized ERYT via the simple evaporation method and simple emulsion. The particle size was determined by scanning electron microscopy and the absence of the interaction with ERYT was confirmed via X-ray diffraction and infrared spectroscopy. The release kinetics of ERYT were studied and then, ERYT loaded PCL and PLGA blends microspheres were used for the inhibition of gram-positive S. aureus strain. The microbial activity was carried out by the agar diffusion disc method. The results show that PCL/PLGA blend and only PCL inhibited the bacteria strain by ERYT present in kinetic aliquots with the complementary effect of the polymers. A numerical model was proposed for modeling the kinetics reported in our study.

Graphical Abstract