Molecular detection of tetB gene in Klebsiella pneumonia, Escherichia coli and association between IL-10 gene polymorphism in burn patients

Mutar, Abbas Dakheel; Degaim, Zainab Dakhil; Jaaz, Widad Sameer; Muhsen, Rahman Kadhum

doi:10.21608/mb.2025.370478.1285

Molecular detection of tetB gene in Klebsiella pneumonia, Escherichia coli and association between IL-10 gene polymorphism in burn patients

Document Type : Original Article

Authors

¹ Animal Production Department, College of Agriculture and Marshes, University of Thi-Qar, Thi-Qar, Iraq.

² Department of Microbiology, College of Medicine, University of Thi-Qar, Thi-Qar, Iraq.

³ Department of Microbiology, College of Medicine, University of Karbala, Karbala, Iraq.

⁴ Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Basra, Iraq.

10.21608/mb.2025.370478.1285

Abstract

This study aimed to check for the tetB gene in K. pneumoniae and E. coli taken from burn wounds and to see if there is a link between IL-10 gene variations and burn patients. One hundred samples of burn wound infections were collected and cultured for isolation and identification of K. pneumoniae and E. coli through morphological characterization, biochemical methods, and API 20E. The PCR technique was used to detect the tetB gene in all K. pneumoniae and E. coli isolates. The present results showed 25% of isolates identified as E. coli, while 80% of isolates identified as K. pneumoniae from burn samples after identification by many tests. The results of the PCR technique documented that 52% of E. coli and 45% of K. pneumoniae isolates harbored the tetB gene. A specific PCR fragment of 781 bp was designed to amplify a specific portion of the IL-10 gene. The current results recorded a genetic polymorphism of the IL-10 gene, rs1800896 (T327C), in burn patients. The results of this study are consistent with HWE with highly significant results (P = 0.000, 0.0002 and 0.000) for all groups, burn and control, respectively.

Keywords

Main Subjects