Phyto-synthesize of selenium nanoparticles via Psidium guajava leaves and study the efficacy versus Methicillin-Resistant Staphylococcus aureus (MRSA) and their biofilm

Ibrahim, Zubydah K.; Al-Haideri, Halah H.

doi:10.21608/mb.2024.334380.1204

Phyto-synthesize of selenium nanoparticles via Psidium guajava leaves and study the efficacy versus Methicillin-Resistant Staphylococcus aureus (MRSA) and their biofilm

Document Type : Original Article

Authors

College of Science for Women, University of Baghdad. Department of Biology, Baghdad, Iraq.

10.21608/mb.2024.334380.1204

Abstract

Selenium nanoparticles (Se NPs) were generated using a green synthesis method utilizing Psidium guajava leaf extract (PGLE). By using FTIR, XRD, SEM, EDX, and DLS, Se NPs have been characterized. According to the results of characterization, Se NPs that have been combined with PGLE show homogenous NP surfaces, a clean appearance, and an average size of 187 nm. From 128 specimens collected from patients with diabetic type 1, only 29 were identified as Staphylococcus species, out of 29 isolates, 25 were identified as S. aureus, and 4 were other Staphylococcus species. MecA gene detection and the cefoxitin resistance pattern were used to identify methicillin-resistant S. aureus MRSA. Of the 25 isolates, 20 were identified as MRSA and 5 as MSSA. Twenty pathogenic MRSA strains were shown to be susceptible to phyto-synthesized SeNPs' antibacterial effects. In addition to demonstrating low MIC values ranging from 50 to 800 μg/mL, the results demonstrated that Se NPs exhibited promising antibacterial activities, with inhibition zones ranging from 12 ± 0.41 to 21.5 ± 0.85 mm. The capacity to form a strong biofilm was seen in only four of the twenty MRSA strains. In tests against four strains known to generate biofilms, the Se NPs proved to be effective. Biosynthesis of Se NPs by PGLE was successful, and these NPs showed promise as an antibacterial and antibiofilm agent against MRSA strains.

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