Isolation of Acetobacter persici DS1 from over-ripened sapodilla fruit as a potential strain for fruit vinegar production

Tripathi, Neha M.; Aseri, Gajender K.; Jain, Amit K.; Sharma, Deepansh; Kaur, Jasmeet; Singh, Deepti

doi:10.21608/mb.2025.310511.1141

Isolation of Acetobacter persici DS1 from over-ripened sapodilla fruit as a potential strain for fruit vinegar production

Document Type : Original Article

Authors

¹ Amity Institute of Microbial Technology, Amity University Rajasthan, Jaipur, Rajasthan, India.

² IGNOU Regional Centre, Karnal, Haryana, India.

³ Department of Life Science, J C Bose University of Science and Technology, YMCA, Faridabad, India.

10.21608/mb.2025.310511.1141

Abstract

The objective of this study was to isolate and identify the acetic acid bacteria (AAB) from over-ripened fruit Sapodilla fruit. The isolated strain was identified by using 16S rRNA sequencing, and strain was designated as Acetobacter persici DS1. The selected strains were utilized to formulate fruit vinegar using two-stage fermentation process using locally obtainable raw fruit processing waste. The strain was found to be a potent strain which can withstand high initial ethanol concentrations and accumulating acetic acid. It is observed that the increase in optical density during the ethanol tolerance or resistance up to 5.5 % (v/v) initial ethanol concentrations and accumulating acetic acid in GYC broth was observed as ethanol and aceto-tolerant strains. The strain A. persici DS1 have substantial growth up to 5.5% (v/v). The strain's maximum enzyme activity was seen at a 10% acetic acid concentration. ADH and ALDH activity of A. persici DS1 were 2.93U mg^-1and 3.52U mg^-1, respectively. According to the final data the acetic acid producing strain A. persici DS1 isolated from sapodilla indicated that the highest acetic acid production 4.63 g/100ml with absolute ethanol and 3.82 g/100ml with distilled ethanol. Therefore, the obtained potential AAB strain can be used for fruit vinegar fermentation at high titration. It can be concluded that the studied strain is well suited to be used as a parental strain to prepare a starter for fruit vinegar production.

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