Influence of Pseudomonas aeruginosa exotoxin A against breast cancer (MCF-7) cell line

Yaseen, Najlaa Nabhan; Hassan, Muthanna Hamid

doi:10.21608/mb.2025.357212.1245

Influence of Pseudomonas aeruginosa exotoxin A against breast cancer (MCF-7) cell line

Document Type : Original Article

Authors

¹ Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq.

² Department of Biology, College of Science, University of Anbar, Anbar, Iraq.

10.21608/mb.2025.357212.1245

Abstract

Out of sixty-nine different clinical samples included UTI, otitis media, wound, and burn infections, fifty samples showed bacterial growth, while the remaining were negative for bacterial growth. Only thirty-one (62%) isolates were related to Pseudomonas aeruginosa. Results indicated that burn infections were the highest with this bacterial colonization. Detection of the exotoxin A was conducted by using an ELISA kit. Current results revealed that, out of thirty-one P. aeruginosa isolates, only twenty-one were able to produce exotoxin A. The isolate (P29) was selected based on its highest productivity of this toxin, reaching 29.24 ng/ml, in addition to partial purification steps for this toxin that had been conducted. The molecular weight of the exotoxin A had been determined and appeared as 65.33 kilodaltons after being compared with standard proteins. The highest concentration of exotoxin A was 400 µg/mL. The results showed that when exotoxin A, which was partially purified, was tested on the MCF-7 cell line for 72 hours at 37°C, it significantly stopped protein production by affecting elongation factor 2 through the action of ADP-ribosyl transferase, but it did not have a noticeable effect on normal human dermal fibroblasts-neonatal (HDFn).

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